Change define region no wrap and assign to proper regions.

templates/layout/region--content.html.twig

-{#
-/**
- * @file
- * Bartik's theme implementation to display a header region.
- *
- * Available variables:
- * - content: The content for this region, typically blocks.
- * - attributes: HTML attributes for the region div.
- * - region: The name of the region variable as defined in the theme's
- *   .info.yml file.
- *
- * @see template_preprocess_region()
- */
-#}
-{% if content %}
-   {{ content }}
-{% endif %}

templates/layout/region--header.html.twig

-{#
-/**
- * @file
- * Bartik's theme implementation to display a header region.
- *
- * Available variables:
- * - content: The content for this region, typically blocks.
- * - attributes: HTML attributes for the region div.
- * - region: The name of the region variable as defined in the theme's
- *   .info.yml file.
- *
- * @see template_preprocess_region()
- */
-#}
-{% if content %}
-   {{ content }}
-{% endif %}

templates/layout/region--primary-menu.html.twig

-{#
-/**
- * @file
- * Bartik's theme implementation to display a header region.
- *
- * Available variables:
- * - content: The content for this region, typically blocks.
- * - attributes: HTML attributes for the region div.
- * - region: The name of the region variable as defined in the theme's
- *   .info.yml file.
- *
- * @see template_preprocess_region()
- */
-#}
-{% if content %}
-   {{ content }}
-{% endif %}

templates/layout/region--sidebar-first.html.twig

-{#
-/**
- * @file
- * Bartik's theme implementation to display a header region.
- *
- * Available variables:
- * - content: The content for this region, typically blocks.
- * - attributes: HTML attributes for the region div.
- * - region: The name of the region variable as defined in the theme's
- *   .info.yml file.
- *
- * @see template_preprocess_region()
- */
-#}
-{% if content %}
-   {{ content }}
-{% endif %}

templates/layout/region--sidebar-second.html.twig

-{#
-/**
- * @file
- * Bartik's theme implementation to display a header region.
- *
- * Available variables:
- * - content: The content for this region, typically blocks.
- * - attributes: HTML attributes for the region div.
- * - region: The name of the region variable as defined in the theme's
- *   .info.yml file.
- *
- * @see template_preprocess_region()
- */
-#}
-{% if content %}
-   {{ content }}
-{% endif %}